Exhibit 23.1
EXHIBIT 23 Consent of Independent Registered Public Accounting Firm
We consent to the incorporation by reference in the Registration Statement (Form S-8 No.
333-84055) pertaining to the Paychex, Inc. 401(k) Incentive Retirement Plan of our report dated
June 24, 2010, with respect to the financial statements and schedule of Paychex, Inc. 401(k)
Incentive Retirement Plan included in this Annual Report (Form 11-K) for the year ended December
31, 2009.
June 24, 2010
Cleveland, Ohio
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